SERTOLI’S CELLS (SC) have been considered for many years to have a simple mechanical architectural function. More recently, these cells, have been re-evaluated with respect to their activity and functions. SC have been recognised to have important functions at different levels, including pathophysiological functions such as the syntesis of androgen binding protein (ABP), the paracrine action on peritubular and Leydig cells. In addition, SC provide an appropriate microenvironment for the development of germ cells. Moreover, recent reports have shown that this interesting cells may provide nutrients, immunomodulatory and trophic factors that are able to ameliorate survival and development of different cell types and improve functional competence both, in vitro and in vivo in different cells. Infact many papers have described the ability of SC to protect transplanted tissue allografts (mainly pancreatic B-cells) by formation of a testis-like immunoprivileged environment, with no need for prolonged immunosuppression therapy regimens.A method for microencapsulation of isolated neonatal porcine Sertoli cells is described. Using conventional alginate-poli-L-ornitina encapsulation procedure, that has been used in our laboratory for almost two decades to embody pancreatic islets, we observed significant loss of sertoli cells viability, possibly due to excessive Ca+2 ion exposure. Replacing calcium with barium, or shortening the incubation period in the presence of Ca ions, we have obtained barium- or calcium-alginate gel micro beads that did not alter morphology and viability of the enveloped Sertoli cells. The procedure may inaugurate a novel approach to help enhance immunological acceptance and implement viability of the cell grafts.